Ms Amila Tikyayala Purnomo¹, Mr Akhmad Noviandi  Syarif¹, Dr Aditya  Wardhana¹, Dr Nurjati  Siregar²

¹Plastic, Reconstructive and Aesthetic Surgery Division, Department of Surgery, Cipto Mangunkusumo Hospital, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, ²Anatomical Pathology Department, Cipto Mangunkusumo Hospital, Faculty of Medicine, Universitas Indonesia , Jakarta, Indonesia

Abstract:

Background:

Burn injury remains a great burden, specifically in Indonesia. In the cases of major burns, xenograft is often used to provide prompt wound coverage. However, some xenografts are not widely available due to cultural, financial, and religious backgrounds or have unesthetic appearance. Striped catfish (Pangasius hypophthalmus) is a scaleless fish that has abundant type 1 collagen. This study aimed to compare striped catfish skin to commonly used xenograft (Nile tilapia and pig skin) as xenograft material for burn wound.

Methods:

This was an experimental study. Skin samples of nine different striped catfishes, Nile tilapias, and pigs were underwent preservation, preparation, and hematoxylin-eosin staining method for histological analysis. Macroscopic and microscopic features of each samples were recorded and analyzed.

Results:

The macroscopic appearance of striped catfishes were hairless and scaleless skin with black-silver color and moderate thickness. As for microscopic features, the epidermal thickness of striped catfishes (8.49±1.60 μm) were significantly different to both Nile tilapias (2.18±0.37 μm; p<0.001) and pigs (42.22±14.85 μm; p=0.002). The dermal thickness of striped catfishes (288.46±119.04 μm) were comparable to Nile tilapias (210.68±46.62 μm; p=0.783) but differs significantly to pigs (1708.44±505.12 μm; p<0.001). The integrity and collagen organization of striped catfishes were comparable to Nile tilapias based on semi-quantitative histology scoring system (p>0.05).

Conclusion:

Striped catfishes had potential macroscopic appearance and comparable microscopic features to Nile tilapia. Therefore, it can be potentially used as a xenograft material. Further studies were required to evaluate the effectiveness and feasibility of striped catfish xenograft in burn wound management.

Keywords:

Xenograft, Striped catfishes (Pangasius hypophthalmus), Nile tilapias (Oreochromis niloticus), Porcine, Burn Wound Coverage

Biography:

5th year Plastic Reconstructive and Aesthetic Surgery in Cipto Mangunkusumo Hospital, Jakarta, Indonesia